Transferability and polymorphism of SSR markers located in flavonoid pathway genes in fragaria and rubus species

Описание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.3390/genes11010011

Ключевые слова: Chitinase, Exons, Flavonoid biosynthesis pathway, Fragaria, Introns, Microsatellites, Polymorphism, Rubus, Transcription factor genes, Transferability

Аннотация: Strawberry (Fragaria) and raspberry (Rubus) are very popular crops, and improving their nutritional quality and disease resistance are important tasks in their breeding programs that are becoming increasingly based on use of functional DNA markers. We identified 118 microsatellite (simple sequence repeat—SSR) loci in the nucleotideПоказать полностьюsequences of flavonoid biosynthesis and pathogenesis-related genes and developed 24 SSR markers representing some of these structural and regulatory genes. These markers were used to assess the genetic diversity of 48 Fragaria and Rubus specimens, including wild species and rare cultivars, which differ in berry color, ploidy, and origin. We have demonstrated that a high proportion of the developed markers are transferable within and between Fragaria and Rubus genera and are polymorphic. Transferability and polymorphism of the SSR markers depended on location of their polymerase chain reaction (PCR) primer annealing sites and microsatellite loci in genes, respectively. High polymorphism of the SSR markers in regulatory flavonoid biosynthesis genes suggests their allelic variability that can be potentially associated with differences in flavonoid accumulation and composition. This set of SSR markers may be a useful molecular tool in strawberry and raspberry breeding programs for improvement anthocyanin related traits. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.

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Издание

Журнал: Genes

Выпуск журнала: Vol. 11, Is. 1

ISSN журнала: 20734425

Издатель: MDPI AG

Авторы

  • Lebedev V.G. (Pushchino State Institute of Natural Sciences, Prospekt Nauki 3, Pushchino, 142290, Russian Federation, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki 6, Pushchino, 142290, Russian Federation)
  • Subbotina N.M. (Pushchino State Institute of Natural Sciences, Prospekt Nauki 3, Pushchino, 142290, Russian Federation, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki 6, Pushchino, 142290, Russian Federation)
  • Maluchenko O.P. (All-Russian Research Institute of Agricultural Biotechnology, Timiriazevskaya Str. 42, Moscow, 127550, Russian Federation)
  • Lebedeva T.N. (Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of Sciences, Institutskaya Str. 2, Pushchino, 142290, Russian Federation)
  • Krutovsky K.V. (Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Göttingen, Büsgenweg 2, Göttingen, 37077, Germany, Center for Integrated Breeding Research, Georg-August University of Göttingen, Albrecht-Thaer-Weg 3, Göttingen, 37075, Germany, Laboratory of Population Genetics, N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkin Str. 3, Moscow, 119333, Russian Federation, Laboratory of Forest Genomics, Genome Research and Education Center, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660036, Russian Federation, Department of Ecosystem Science and Management, Texas A&M University, 2138 TAMU, College Station, TX 77843-2138, United States)
  • Shestibratov K.A. (Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki 6, Pushchino, 142290, Russian Federation)

Вхождение в базы данных

  • Scopus