Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug

Описание

Тип публикации: статья из журнала

Год издания: 2013

Идентификатор DOI: 10.1007/s10856-013-4941-2

Ключевые слова: 3-hydroxybutyric acid, Average diameter, Cell attachments, Chemical compositions, Mass concentration, Mouse-fibroblasts, Polyhydroxyalkanoates, Solvent evaporation techniques, Biocompatibility, Cell culture, Cells, Loading, 3 hydroxybutyric acid, 3 hydroxyhexanoic acid, 4 hydroxybutyric acid, 4',6 diamidino 2 phenylindole, DNA, doxorubicin, nanoparticle, polyhydroxyalkanoic acid, polymer, polystyrene, solvent, unclassified drug, animal cell, article, biocompatibility, biodegradability, cell adhesion, cell proliferation, cell strain 3T3, cell viability, chemical composition, chemical structure, controlled study, cytotoxicity, drug efficacy, drug release, electrophoretic mobility, encapsulation, evaporation, fibroblast, in vitro study, nonhuman, particle size, priority journal, stain, study, surface charge, zeta potential, Absorbable Implants, Animals, Cytostatic Agents, Doxorubicin, Drug Carriers, Drug Compounding, Drug Evaluation, Preclinical, Materials Testing, Mice, Microscopy, Electron, Scanning, Microspheres, Models, Biological, NIH 3T3 Cells, Particle Size, Polyhydroxyalkanoates, Polymers

Аннотация: Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric and 3-hydroxyvaleric acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoic acids (P3HB/3HHx) were prepared usiПоказать полностьюng the solvent evaporation technique, from double emulsions. The study addresses the influence of the chemical compositions on the size and xi-potential of microparticles. P3HB microparticles loaded with doxorubicin have been prepared and investigated. Their average diameter and xi-potential have been found to be dependent upon the level of loading (1, 5, and 10 % of the polymer mass). Investigation of the in vitro drug release behavior showed that the total drug released from the microparticle into the medium increased with mass concentration of the drug. In this study mouse fibroblast NIH 3T3 cells were cultivated on PHA microparticles, and results of using fluorescent DAPI DNA stain, and MTT assay showed that microparticles prepared from PHAs of different chemical compositions did not exhibit cytotoxicity to cells cultured on them and proved to be highly biocompatible. Cell attachment and proliferation on PHA microparticles were similar to those on polystyrene. The cytostatic drug encapsulated in P3HB/3HV microparticles has been proven to be effective against HeLa tumor cells.

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Издание

Журнал: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE

Выпуск журнала: Vol. 24, Is. 8

Номера страниц: 1905-1915

ISSN журнала: 09574530

Место издания: DORDRECHT

Издатель: SPRINGER

Авторы

  • Murueva A.V. (Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Modern Biol & Biotechnol, Krasnoyarsk 660041, Russia)
  • Shishatskaya E.I. (Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Modern Biol & Biotechnol, Krasnoyarsk 660041, Russia)
  • Kuzmina A.M. (Siberian Fed Univ, Inst Modern Biol & Biotechnol, Krasnoyarsk 660041, Russia)
  • Volova T.G. (Inst Biophys SB RAS, Krasnoyarsk 660036, Russia; Siberian Fed Univ, Inst Modern Biol & Biotechnol, Krasnoyarsk 660041, Russia)
  • Sinskey A.J. (MIT, Dept Biol, Cambridge, MA 02139 USA; MIT, Engn Syst Div, Cambridge, MA 02139 USA; MIT, Hlth Sci Technol Div, Cambridge, MA 02139 USA)

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