Тип публикации: доклад, тезисы доклада, статья из сборника материалов конференций
Конференция: International Multiconference on Bioinformatics of Genome Regulation and Structure\Systems Biology (BGRS\SB); Novosibirsk, RUSSIA; Novosibirsk, RUSSIA
Год издания: 2019
Идентификатор DOI: 10.1186/s12870-019-1637-x
Ключевые слова: Cytoplasmic male sterility, Sunflower, Mitochondrial genome rearrangements, CMS MAX1, atp6 chimeric gene, SunflowerPlant Proteins
Аннотация: Background: More than 70 cytoplasmic male sterility (CMS) types have been identified in Helianthus, but only for less than half of them, research of mitochondrial organization has been conducted. Moreover, complete mitochondrion sequences have only been published for two CMS sources - PET1 and PET2. It has been demonstrated that otПоказать полностьюher sunflower CMS sources like MAX1, significantly differ from the PET1 and PET2 types. However, possible molecular causes for the CMS induction by MAX1 have not yet been proposed. In the present study, we have investigated structural changes in the mitochondrial genome of HA89 (MAX1) CMS sunflower line in comparison to the fertile mitochondrial genome. Results: Eight significant major reorganization events have been determined in HA89 (MAX1) mtDNA: one 110 kb inverted region, four deletions of 439 bp, 978 bp, 3183 bp and 14,296 bp, respectively, and three insertions of 1999 bp, 5272 bp and 6583 bp. The rearrangements have led to functional changes in the mitochondrial genome of HA89 (MAX1) resulting in the complete elimination of orf777 and the appearance of new ORFs - orf306, orf480, orf645 and orf1287. Aligning the mtDNA of the CMS sources PET1 and PET2 with MAX1 we found some common reorganization features in their mitochondrial genome sequences. Conclusion: The new open reading frame orf1287, representing a chimeric atp6 gene, may play a key role in MAX1 CMS phenotype formation in sunflower, while the contribution of other mitochondrial reorganizations seems to appear negligible for the CMS development. Background: More than 70 cytoplasmic male sterility (CMS) types have been identified in Helianthus, but only for less than half of them, research of mitochondrial organization has been conducted. Moreover, complete mitochondrion sequences have only been p
Журнал: BMC PLANT BIOLOGY
Выпуск журнала: Vol. 19
Номера страниц: 51
ISSN журнала: 14712229
Место издания: LONDON
Издатель: BMC