Тип публикации: статья из журнала
Год издания: 2015
Идентификатор DOI: 10.1134/S1607672915060216
Аннотация: A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored pПоказать полностьюroduct after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro.
Журнал: DOKLADY BIOCHEMISTRY AND BIOPHYSICS
Выпуск журнала: Vol. 465, Is. 1
Номера страниц: 428-431
ISSN журнала: 16076729
Место издания: NEW YORK
Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER