Cloning and molecular organization of the polyhydroxyalkanoic acid synthase gene (phaC) of Ralstonia eutropha strain B5786

Описание

Тип публикации: статья из журнала

Год издания: 2010

Идентификатор DOI: 10.1134/S0003683810020031

Ключевые слова: Aeromonas punctata, Cupriavidus necator, Ectothiorhodospira shaposhnikovii, Pseudomonas, Pseudomonas sp. 61-3, Rhodococcus, Rhodococcus ruber, Rhodospirillum rubrum, Thiococcus pfennigii

Аннотация: Class I polyhydroxyalkanoic acid (PHA) synthase gene (phaC) of Ralstonia eutropha strain B5786 was cloned and characterized. R. eutropha B5786 features the ability to synthesize multicomponent PHAs with short- and medium-chain-length monomers from simple carbohydrate substrate. A correlation was made between the molecular structureПоказать полностьюof PHA synthase and substrate specificity and the ability of strain-producers to accumulate PHAs of this or that structure. A strong similarity of PHA synthase of R. eutropha strain B5786 with PHA synthase of R. eutropha strain H16, which, as opposed to strain B5786, enables to incorporate medium chain length PHAs if hexanoate is used as carbon source, exhibited 99%. A correlation between the structure of PHA synthase of B5786 strain with synthases of microorganisms which synthesize short and medium chain length PHAs similarly to B5786 strain, showed an identity level from 26 to 41% (homology with synthase of Rhodospirillum rubrum makes 41%, Ectothiorhodospira shaposhnikovii makes 26%, Aeromonas punctata makes 40%, Thiococcus pfennigii makes 28%, Rhodococcus ruber makes 38%, and with PhaCl and PhaC2 synthases of Pseudomonas sp. 61-3 makes 34 and 37%, respectively). This allows for speaking about the absence of a direct connection between the molecular organization of PHA synthases and their functional abilities, namely, the ability to synthesize PHAs of a particular composition.

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Издание

Журнал: APPLIED BIOCHEMISTRY AND MICROBIOLOGY

Выпуск журнала: Vol. 46, Is. 2

Номера страниц: 140-147

ISSN журнала: 00036838

Место издания: NEW YORK

Издатель: MAIK NAUKA/INTERPERIODICA/SPRINGER

Авторы

  • Kozhevnikov I.V. (Siberian Federal University)
  • Volova T.G. (Institute of Biophysics of Siberian Branch,Russian Academy of Sciences)
  • Hai T. (Institute of Molecular Microbiology and Biotechnology)
  • Steinbuchel A. (Institute of Molecular Microbiology and Biotechnology)

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