Sulfated derivatives of arabinogalactan and their anticoagulant activity

Описание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.14258/jcprm.2019044845

Ключевые слова: anticoagulant activity, molecular weight, sulfated arabinogalactan, sulfur content

Аннотация: The IR spectra and molecular mass distribution of arabinogalactan sulfates in the form of sodium and ammonium salts, obtained using various sulfating reagents, were compared. According to the obtained data, the sulfated derivatives of arabinogalactan differ from each other by the nature of the hydrogen bonds and the molecular weighПоказать полностьюt distribution. Using coagulological tests at the activation of coagulation of platelet-poor human plasma, in vitro study of the anticoag-ulant properties of arabinogalactan sulfated derivatives in various salt forms differing in methods of preparation, degree of sul-fation and molecular weight was conducted. It was established that the sample in the form of arabinogalactan sodium salt (SAG 1) with a sulfur content of 13.2 wt.% ?nd a polydispersity degree of 1.52 showed 2 times more anticoagulant activity than the sample in the form of ammonium salt of arabinogalactan (SAG 2) with the sulfur content of 6.6 wt.% and the degree of polydispersity of 1.30. Antithrombin (aIIa) activity of samples obtained by sulfation with pyridine and sulfuric anhydride complex (SAG 1) and sulfamic acid complex (SAG 2) was, respectively, 23.42±1.86 and 10.20±1.50 U/mg; the anti-factor Xa activity of SAG 1 and SAG 2 was 2.13±0.42 and 0.37±0.08 U/mg; and the ratio aIIa/aXa for SAG1 and SAG 2 was 11 and 28, respectively. The less activity of antifactor Xa(aXA) of SAG as compared to unfractionated heparin (UFG) and higher rations of ac-tivities aIIa/aXa may contribute the less provocation of bleeding by SAG samples in the comparition with UFG. © 2020 Altai State University. All rights reserved.

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Издание

Журнал: Khimiya Rastitel'nogo Syr'ya

Выпуск журнала: Is. 4

Номера страниц: 47-56

ISSN журнала: 10295151

Авторы

  • Kuznetsova S.A. (Institute of Chemistry and Chemical Technology, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Academgorodok 50/24, Krasnoyarsk, 660036, Russian Federation)
  • Vasilyeva N.Yu. (Institute of Chemistry and Chemical Technology, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Academgorodok 50/24, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, pr. Svobodny, 79, Krasnoyarsk, 660049, Russian Federation)
  • Drozd N.N. (FGBU, National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Novy Zykovsky Proezd, 4, Moscow, 125167, Russian Federation)
  • Mikhailenko M.A. (Institute of Solid State Chemistry and Mechanochemistry, SB, RAS, ul. Kutateladze, 18, Novosibirsk, 630128, Russian Federation)
  • Shakhtshneider T.P. (Institute of Solid State Chemistry and Mechanochemistry, SB, RAS, ul. Kutateladze, 18, Novosibirsk, 630128, Russian Federation, Novosibirsk State University, ul. Pirogova, 2, Novosibirsk, 630090, Russian Federation)
  • Malyar Yu.N. (Institute of Chemistry and Chemical Technology, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Academgorodok 50/24, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, pr. Svobodny, 79, Krasnoyarsk, 660049, Russian Federation)
  • Kuz-Netsov B.N. (Institute of Chemistry and Chemical Technology, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Academgorodok 50/24, Krasnoyarsk, 660036, Russian Federation, Siberian Federal University, pr. Svobodny, 79, Krasnoyarsk, 660049, Russian Federation)
  • Chesnokov N.V. (Institute of Chemistry and Chemical Technology, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Academgorodok 50/24, Krasnoyarsk, 660036, Russian Federation)

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