Photo-immobilization of proteins on carbons

Описание

Тип публикации: статья из журнала

Год издания: 2020

Идентификатор DOI: 10.1016/j.jphotobiol.2019.111675

Ключевые слова: Bovine serum albumin, Carbons, Graphene oxide, Graphene oxide modified with SO2, Graphite, Photo-immobilization, α-amylaseamylase, 9000-90-2, 9000-92-4, 9001-19-8, carbon, 7440-44-0, graphite, 7782-42-5, protein, 67254-75-5

Аннотация: The photofunctionalization of three different carbons with two proteins was studied at room temperature. Water solutions of bovine serum albumin, BSA, and α-amylase, AA, were photolyzed at 21 °C in the presence of graphite microparticles (6.20 μm), MPG, graphene oxide, MPGO, and graphene oxide modified with SO2, mMPGO. The insertioПоказать полностьюn of BSA on carbon matrixes occurred with a deoxygenation reaction, most likely due to a dehydration step of a water molecule. XPS, TOC and TGA, showed that the BSA photo-insertion on MPG was highly efficient with 34.9% of the weight of MPG after photolysis, with an initial concentration of 1 g∙L−1 of BSA. A high yield of AA photoinsertion on the carbons was also obtained. The calculated weight of AA inserted on MPG and MPGO after photolysis was 22.30% and 18.08%, respectively, with respect to the initial weight of carbon, when the initial concentration of AA was 60 mg∙L−1. AA immobilized on MPG was active while the enzyme on MPGO showed a smaller activity, within the experimental error. Although a certain extent of denaturalization of both proteins was observed during photolysis, the molecular weight and composition changed very little during the photolysis, which would produce mainly conformational changes and isomerization reactions. © 2019 Elsevier B.V.

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Издание

Журнал: Journal of Photochemistry and Photobiology B: Biology

Выпуск журнала: Vol. 202

ISSN журнала: 10111344

Издатель: Elsevier B.V.

Авторы

  • Humeres E. (Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil)
  • Canle M. (Universidade da Coruña, Grupo Reactividade Química e Fotorreactividade (React!), Departamento de Química, Facultade de Ciencias & CICA, A Coruña, E-15071, Spain)
  • Lopes C.N. (Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil)
  • Santaballa J.A. (Universidade da Coruña, Grupo Reactividade Química e Fotorreactividade (React!), Departamento de Química, Facultade de Ciencias & CICA, A Coruña, E-15071, Spain)
  • Debacher N.A. (Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil)
  • Moreira R.D.F.P.M. (Departamento de Engenharia Química e de Alimentos, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil)
  • Safin V. (Universidade da Coruña, Grupo Reactividade Química e Fotorreactividade (React!), Departamento de Química, Facultade de Ciencias & CICA, A Coruña, E-15071, Spain, Department of Chemistry and Technology of Natural Energy Carriers and Carbon Materials, Siberian Federal University, Russian Federation)
  • Pérez M.I.F. (Universidade da Coruña, Grupo Reactividade Química e Fotorreactividade (React!), Departamento de Química, Facultade de Ciencias & CICA, A Coruña, E-15071, Spain)

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