Bioluminescent monitoring of detoxification processes: Activity of humic substances in quinone solutions

Описание

Тип публикации: статья из журнала

Год издания: 2007

Идентификатор DOI: 10.1016/j.jphotobiol.2007.05.007

Ключевые слова: bioluminescent assay; detoxification; humic substances; quinones, Bioluminescent assay, Detoxification, Humic substances, Quinones, 1,4 benzoquinone, 1,4 benzoquinone derivative, 1,4 naphthoquinone, environmental chemical, flavine mononucleotide reductase, fluoranil, methyl 1,4 benzoquinone derivative, quinone derivative, reduced nicotinamide adenine dinucleotide, tetramethyl 1,4 benzoquinone, unclassified drug, absorption spectroscopy, article, bacterial cell, bioassay, bioluminescence, cell membrane, controlled study, ecotoxicity, electron microscopy, enzyme isolation, humic substance, in vitro study, in vivo study, nonhuman, oxidation reduction reaction, Photobacterium phosphoreum, photodegradation, priority journal, spectrometry, FMN Reductase, Luciferases, Bacterial, Luminescent Measurements, Oxidation-Reduction, Photobacterium, Solutions, Spectrophotometry, Bacteria (microorganisms)

Аннотация: This study deals with application of bioluminescent assay systems to evaluate the detoxifying effect of humic substances (HS) on the solutions of organic oxidizers - quinones. A series of homologous quinones with different redox characteristics: 1,4-benzoquinone, fetrafluoro-1,4-benzoquinone, methyl-1,4-benzoquinone, tetramethyl-1,Показать полностью4-benzoquinone, and -1,4-naphtoquinone, was used. Bioluminescent bacteria Photobacterium phosphoreum, and NADH:FMN-oxidoreductase-luciferase enzyme system isolated from these bacteria were used as assay systems. The toxicity was compared in the presence and in the absence of HS. Variation of complexity of bioassays (in vivo or in vitro) combined with spectrometric and microscopic methods, provides insight into the process of detoxification in quinone solutions. Two ways of HS effect were studied: the reduction activity of HS and intensification of self-protection of bacterial cells on HS addition. (C) 2007 Published by Elsevier B.V.

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Издание

Журнал: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY

Выпуск журнала: Vol. 88, Is. 2-3

Номера страниц: 131-136

ISSN журнала: 10111344

Место издания: LAUSANNE

Издатель: ELSEVIER SCIENCE SA

Авторы

  • Fedorova E. (Siberian Federal University)
  • Kudryasheva N. (Siberian Federal University)
  • Kuznetsov A. (Institute of Biophysics SB RAS)
  • Mogil'naya O. (Institute of Biophysics SB RAS)
  • Stom D. (Irkutsk State University,Biology Research Institute)

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