Тип публикации: статья из журнала
Год издания: 2017
Ключевые слова: Antibiotic resistance, Klebsiella pneumoniae, Neutrophilic granulocytes, Phagocytosis, Pseudomonas aeruginosa, Staphylococcus aureus
Аннотация: The study investigates the phagocytic activity of neutrophilic granulocytes under the influence of antibiotic-resistant strains of bacteria Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneimoniae belonging to extended-spectrum -lactamases. The subjects of the study were neutrophilic granulocytes of blood isolated fПоказать полностьюrom healthy people and strains of bacteria S.aureus, P.aeruginosa, and K.pneumoniae resistant and sensitive to antibiotics. The functions of phagocytosis (phagocytic number and phagocytic index) were assessed with the help of Fl'l'C (Fluoresceinlsothyocyanate) - labeled bacteria. The analysis of stained cells was carried out on flow cytofluorimeter FC-500 (BeckmanCoulter, USA) in whole peripheral blood. A different phagocytic response of neutrophils to resistant and sensitive strains of bacteria was detected. Thus, in response to resistant strains of S.aureus, the percentage of neutrophils entering phagocytosis and the average number of intracellular bacteria increases with respect to sensitive strains. In response to resistant strains of K.pneumonia, the phagocytic activity of neutrophilic granulocytes decreases. When neutrophil granulocytes were induced, resistant strains of P.aeruginosa did not show significant differences with respect to sensitive strains. © 2017 Media Sphera Publishing Group. All rights reserved.
Журнал: Antibiotiki i Khimioterapiya
Выпуск журнала: Vol. 62, Is. 9-10
Номера страниц: 15-18
ISSN журнала: 02352990
Издатель: Media Sphera Publishing Group