Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.3390/ijms18081685

Ключевые слова: Acetylcholinesterase inhibition, Calendula officinalis, HPLC, Isorhamnetin, Marigold, Quercetinisorhamnetin, 480-19-3

Аннотация: Marigold (Calendula officinalis L.) is one of the most common and widespread plants used medicinally all over the world. The present study aimed to evaluate the anti-acetylcholinesterase activity of marigold flowers, detect the compounds responsible and perform chemical analysis of marigold commercial products. Analysis of 23 varieПоказать полностьюties of C. officinalis flowers introduced into Siberia allowed us to select the Greenheart Orange variety due to the superior content of flavonoids (46.87 mg/g) and the highest inhibitory activity against acetylcholinesterase (IC50 63.52 µg/mL). Flavonoids, isorhamnetin and quercetin derivatives were revealed as potential inhibitors with the application of high-performance liquid chromatography (HPLC) activity-based profiling. Investigation of the inhibitory activity of isorhamnetin glycosides demonstrated the maximal potency for isorhamnetin-3-O-(2″,6″-di-acetyl)-glucoside (IC50 51.26 µM) and minimal potency for typhaneoside (isorhamnetin-3-O-(2″,6″-di-rhamnosyl)-glucoside; IC50 94.92 µM). Among quercetin derivatives, the most active compound was quercetin-3-O-(2″,6″-di-acetyl)-glucoside (IC50 36.47 µM), and the least active component was manghaslin (quercetin-3-O-(2″,6″-di-rhamnosyl)-glucoside; IC50 94.92 µM). Some structure-activity relationships were discussed. Analysis of commercial marigold formulations revealed a reduced flavonoid content (from 7.18-19.85 mg/g) compared with introduced varieties. Liquid extract was the most enriched preparation, characterized by 3.10 mg/mL of total flavonoid content, and infusion was the least enriched formulation (0.41 mg/mL). The presented results suggest that isorhamnetin and quercetin and its glycosides can be considered as potential anti-acetylcholinesterase agents. © 2017 by the authors. Licensee MDPI, Basel, Switzerland.

Журнал: International Journal of Molecular Sciences

Выпуск журнала: Vol. 18, Is. 8

Номера страниц: 1685

ISSN журнала: 16616596

Издатель: MDPI AG

• Olennikov D.N. (Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Sakh’yanovoy Street 6, Ulan-Ude, Russian Federation, Department of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Street, Yakutsk, Russian Federation)
• Kashchenko N.I. (Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, Sakh’yanovoy Street 6, Ulan-Ude, Russian Federation)
• Chirikova N.K. (Department of Biochemistry and Biotechnology, North-Eastern Federal University, 58 Belinsky Street, Yakutsk, Russian Federation)
• Akobirshoeva A. (Pamir Biological Institute, Kholdorova Street 1, Khorog, Tajikistan)
• Zilfikarov I.N. (All-Russian Institute of Medical and Aromatic Plants, Greena Street 7/1, Moscow, Russian Federation)
• Vennos C. (Regulatory and Medical Scientific Affairs, Padma AG, 1 Underfeldstrasse, Hinwil, Switzerland)

• Scopus