Reaction rates in enzymatic assay system in solutions of metal salts and humic substances

Описание

Тип публикации: глава из книги

Год издания: 2013

Идентификатор DOI: 10.1007/978-94-007-5634-2_101

Ключевые слова: Bioluminescent assay, Enzyme reaction rates, Humic substances, Metal salts, Toxicity

Аннотация: This chapter considers the effects of humic substances (HS) on solutions of model inorganic pollutants, metal salts - K3[Fe(CN)6] and CuSO4. Bioluminescent system of coupled enzymatic reactions catalyzed by NAD(P)H-FMN oxidoreductase and bacterial luciferase was used as a bioassay to monitor toxicity of salt solutions in the presenПоказать полностьюce and absence of HS. Ability of HS to decrease or increase general toxicity of the solutions was revealed. The rates of redox reaction in bioluminescent assay system were analyzed in the absence and presence of HS. Acceleration of NADH auto-oxidation by HS was supposed to result in toxicity increase. Acceleration of endogenous NADH-dependent redox reactions in the bioluminescent assay system is supposed to be responsible for detoxification of the salt solutions. © 2013 Zhejiang University Press and Springer Science+Business Media Dordrecht. All rights are reserved.

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Издание

Журнал: FUNCTIONS OF NATURAL ORGANIC MATTER IN CHANGING ENVIRONMENT

Выпуск журнала: Vol. 9789400756342

Номера страниц: 561-565

Авторы

  • Tarasova A.S. (Siberian Federal University)
  • Kudryasheva N.S. (Institute of Biophysics SB RAS)

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