Genetically encodable bioluminescent system from fungi

Описание

Тип публикации: статья из журнала

Год издания: 2018

Идентификатор DOI: 10.1073/pnas.1803615115

Ключевые слова: bioluminescence, Fungal luciferase, Fungal luciferin biosynthesis

Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis haПоказать полностьюs been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering.

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Издание

Журнал: Proceedings of the National Academy of Sciences of the United States of America

Выпуск журнала: Т.115, 50

Номера страниц: 12728-12732

ISSN журнала: 00278424

Издатель: Proquest Academic Research Library

Авторы

  • Kotlobay A.A. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Sarkisyan K.S. (Medical Research Council London Institute of Medical Sciences,Imperial College London)
  • Mokrushina Y.A. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Serebrovskaya E.O. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Markina N.M. (Evrogen JSC)
  • Gorokhovatsky A.Y. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Vvedensky A. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Chepurnyh T.V. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Ziganshin R. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Tsarkova A.S. (Pirogov Russian National Research Medical University)
  • Kaskova Z.M. (Pirogov Russian National Research Medical University)
  • Shender V. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Eroshkin F.M. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Zaraisky A.G. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Mishin A.S. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Dolgov S.V. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Mitiouchkina T.Y. (Planta LLC)
  • Kopantzev E.P. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Barsova E. (Evrogen JSC)
  • Bogdanova E.A. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Lukyanov S. (Pirogov Russian National Research Medical University)
  • Smirnov I.V. (Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences)
  • Yampolsky I.V. (Pirogov Russian National Research Medical University)
  • Somermeyer L.G. (Institute of Science and Technology Austria)
  • Kondrashov F.A. (Institute of Science and Technology Austria)
  • Marcet-Houben M. (Universitat Pompeu Fabra)
  • Gabaldón T. (Catalan Institution for Research and Advanced Studies (ICREA))
  • Purtov K.V. (Institute of Biophysics,Federal Research Center Krasnoyarsk Science Center,Siberian Branch,Russian Academy of Sciences)
  • Petushkov V.N. (Institute of Biophysics,Federal Research Center Krasnoyarsk Science Center,Siberian Branch,Russian Academy of Sciences)
  • Rodionova N.S. (Institute of Biophysics,Federal Research Center Krasnoyarsk Science Center,Siberian Branch,Russian Academy of Sciences)
  • Gitelson J.I. (Institute of Biophysics,Federal Research Center Krasnoyarsk Science Center,Siberian Branch,Russian Academy of Sciences)
  • Fakhranurova L.I. (Institute of Theoretical and Experimental Biophysics,Russian Academy of Sciences)
  • Guglya E.B. (Pirogov Russian National Research Medical University)
  • Abakumov M. (Biomedical Nanomaterials,National Research Technological University (MISiS))
  • Povolotskaya I.S. (Pirogov Russian National Research Medical University)
  • Abakumova T.O. (Skolkovo Institute of Science and Technology)
  • Waldenmaier H.E. (Departamento de Bioquímica,Instituto de Química,Universidade de São Paulo)
  • Oliveira A.G. (Departamento de Oceanografia Física,Química e Geológica,Instituto Oceanográfico,Universidade de São Paulo)
  • Oba Y. (Department of Environmental Biology,Chubu University)
  • Stevani C.V. (Departamento de Química Fundamental,Instituto de Química,Universidade de São Paulo)

Вхождение в базы данных

  • РИНЦ (eLIBRARY.RU) (цитирований 2)
  • Ядро РИНЦ (eLIBRARY.RU)
  • Web of Science Core Collection
  • Scopus