Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics

Описание

Тип публикации: статья из журнала

Год издания: 2021

Идентификатор DOI: 10.1016/j.chemosphere.2020.128180

Ключевые слова: metabolic activity, p(3hb) bioplastic, p(3hb) properties, p(3hb)-degrading strains, siberian cryogenic soils, structure of microbial communitythis study (polymer synthesis and investigation) was financially supported by project “agropreparations of the new generation: a strategy of construction and realization” (agreement no 074-02-2018-328) in accordance with resolution no 220 of the government of the russian federation of april 9, 2010, “on measures designed to attract leading scientists to the russian institutions of higher learning”, and by the state assignment of the ministry of science and higher education of the russian federation no. fsrz-2020-0006 (investigation of polymer degradation in soils of evenkia).

Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5Показать полностью-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g−1 soil and CO2 production was 20–46 mg g−1 day−1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) × 103 and (18.3 ± 2.2) × 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) × 106 CFU g−1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd

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Издание

Журнал: Chemosphere

Выпуск журнала: Vol. 263

Номера страниц: 128180

ISSN журнала: 00456535

Авторы

  • Prudnikova S.V. (Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation)
  • Evgrafova S.Y. (Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation, V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation, Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation)
  • Volova T.G. (Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation)

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