Тип публикации: статья из журнала
Год издания: 2000
Аннотация: Eect of dielectric properties of media on kinetic parameters of bioluminescent reaction?of luciferase of Vibrio harveyi was studied with addition of organic solvents (ethanol,?methanol, acetone, ethylene glycol, formamide and dimethyl sulphoxide). Small concentration?of organic solvents (0.5{18% v/v) can both activate and inhibit iПоказать полностьюntensity of?luminescence depending on the their physical-chemical properties, higher concentrations?inhibit bioluminscence in vitro. The interaction of kinetic parameters of bioluminescent?reaction and dielectric permittivity of the medium " cannot be described by a unied?dependence. With addition of solvents when " ranges from 74 to 82 increase of the?maximum reaction rate depends on the balance between the capacity of the solvent to?form hydrogen bonds and moderate hydrophobic contacts. Addition of formamide attenuates?electrostatic interactions and increases the decay rate of excited emitter. High?(compared to alcohols) electron-donor capacity of DMSO and acetone helps stabilize the?excited intermediate of the reaction.
Журнал: Вестник Московского университета. Серия 2: Химия
Выпуск журнала: Т. 41, № S6
Номера страниц: 8-11
ISSN журнала: 05799384
Место издания: Москва
Издатель: Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" Издательский Дом (типография)