Variation of Spectral Characteristics of Coelenteramide-Containing Fluorescent Protein from Obelia Longissima Exposed to Dimethyl Sulfoxide

Описание

Тип публикации: статья из журнала

Год издания: 2016

Идентификатор DOI: 10.1007/s11182-016-0806-8

Ключевые слова: fluorescent coelenteramide-containing fluorescent proteins, discharged obelin, proton transfer, dimethyl sulfoxide

Аннотация: Effect of dimethyl sulfoxide (DMSO), a widespread biomedical agent, on spectral-luminescent characteristics of coelenteramide-containing fluorescent protein - discharged obelin - is investigated. Contributions of violet and blue-green spectral components to fluorescence of discharged obelin are elucidated and characterized at diffeПоказать полностьюrent photoexcitation energies. Dependences of these contributions on the DMSO concentration are presented. Spectral changes are related to the destructive effect of DMSO on fluorescent protein and decreasing efficiency of proton transfer to electronically excited states of fluorophore.

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Издание

Журнал: RUSSIAN PHYSICS JOURNAL

Выпуск журнала: Vol. 59, Is. 4

Номера страниц: 562-567

ISSN журнала: 10648887

Место издания: NEW YORK

Издатель: SPRINGER

Авторы

  • Petrova A.S. (Krasnoyarsk State Agrarian University)
  • Alieva R.R. (Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences)
  • Belogurova N.V. (Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences)
  • Tirranen L.S. (Krasnoyarsk State Agrarian University)
  • Kudryasheva N.S. (Siberian Federal University)

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