Mechanism and color modulation of fungal bioluminescence

Описание

Тип публикации: статья из журнала

Год издания: 2017

Идентификатор DOI: 10.1126/sciadv.1602847

Ключевые слова: SINGLET MOLECULAR-OXYGEN, Quantum yields, chemiluminescence, decomposition, endoperoxide, peroxides, emission, light

Аннотация: Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fПоказать полностьюungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates.

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Издание

Журнал: Science Advances

Выпуск журнала: Т.3, 4

Номера страниц: 1602847

ISSN журнала: 23752548

Авторы

  • Kaskova Zinaida M. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Dörr Felipe A. (Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicolgicas, BR-05508900 Sao Paulo, Brazil)
  • Petushkov Valentin N. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Purtov Konstantin V. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Tsarkova Aleksandra S. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Rodionova Natalja S. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Mineev Konstantin S. (Russian Acad Sci, Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117997, Russia)
  • Guglya Elena B. (Pirogov Russian Natl Res Med Univ, OStrovitianov 1, Moscow 117997, Russia)
  • Kotlobay Alexey (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Baleeva Nadezhda S. (Pirogov Russian Natl Res Med Univ, OStrovitianov 1, Moscow 117997, Russia)
  • Baranov Mikhail S. (Pirogov Russian Natl Res Med Univ, OStrovitianov 1, Moscow 117997, Russia)
  • Arseniev Alexander S. (Russian Acad Sci, Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117997, Russia)
  • Gitelson Josef I. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)
  • Lukyanov Sergey (Pirogov Russian Natl Res Med Univ, OStrovitianov 1, Moscow 117997, Russia)
  • Suzuki Yoshiki (Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan)
  • Kanie Shusei (Nagoya Univ, Grad Sch Bioagr Sci, Nagoya, Aichi 4648601, Japan)
  • Pinto Ernani (Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicolgicas, BR-05508900 Sao Paulo, Brazil)
  • Di Mascio Paolo (Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508900 Sao Paulo, Brazil)
  • Waldenmaier Hans E. (Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil)
  • Pereira Tatiana A. (Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil)
  • Carvalho Rodrigo P. (Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil)
  • Oliveira Anderson G. (Univ Sao Paulo, Inst Oceanografico, Dept Oceanografia Fis Quim & Geol, BR-05508120 Sao Paulo, Brazil)
  • Oba Yuichi (Chubu Univ, Dept Environm Biol, Kasugai, Aichi 4878501, Japan)
  • Bastos Erick L. (Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil)
  • Stevani Cassius V. (Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508900 Sao Paulo, Brazil)
  • Yampolsky Ilia V. (SB RAS, Fed Res Ctr Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Russia)

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