Тип публикации: статья из журнала
Год издания: 2017
Идентификатор DOI: 10.1126/sciadv.1602847
Ключевые слова: SINGLET MOLECULAR-OXYGEN, Quantum yields, chemiluminescence, decomposition, endoperoxide, peroxides, emission, light
Аннотация: Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fПоказать полностьюungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates.
Журнал: Science Advances
Выпуск журнала: Т. 3, № 4
Номера страниц: 1602847
ISSN журнала: 23752548