Тип публикации: статья из журнала
Год издания: 2022
Идентификатор DOI: 10.3390/ijms23095152
Ключевые слова: antioxidant activity, bioluminescence bioassay, endohedral fullerenol, gadolinium, hormesis, oxidative stress, reactive oxygen species, toxicity
Аннотация: The Gd-containing metallofullerene derivatives are perspective magnetic resonance imaging contrast agents. We studied the bioeffects of a water-soluble fullerene derivative, gadolinium-endohedral fullerenol, with 40–42 oxygen groups (Gd@Fln). Bioluminescent cellular and enzymatic assays were applied to monitor toxicity and antioxidПоказать полностьюant activity of Gd@Fln in model solutions; bio-luminescence was applied as a signaling physiological parameter. The Gd@Fln inhibited biolumi-nescence at high concentrations (>2∙10−1 gL−1), revealing lower toxicity as compared to the previously studied fullerenols. Efficient activation of bioluminescence (up to almost 100%) and consumption of reactive oxygen species (ROS) in bacterial suspension were observed under low-concentration exposure to Gd@Fln (10−3–2∙10−1 gL−1). Antioxidant capability of Gd@Fln was studied under conditions of model oxidative stress (i.e., solutions of model organic and inorganic oxidizers); antioxidant coefficients of Gd@Fln were determined at different concentrations and times of exposure. Contents of ROS were evaluated and correlations with toxicity/antioxidant coefficients were determined. The bioeffects of Gd@Fln were explained by hydrophobic interactions, electron affinity, and disturbing of ROS balance in the bioluminescence systems. The results contribute to understanding the molecular mechanism of “hormetic” cellular responses. Advantages of the bioluminescence assays to compare bioeffects of fullerenols based on their structural characteristics were demonstrated. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Журнал: International Journal of Molecular Sciences
Выпуск журнала: Vol. 23, Is. 9
Номера страниц: 5152
ISSN журнала: 16616596
Издатель: MDPI